California's iconic Laguna Seca venue will be missing from the MotoGP World Championship calendar next season.
A statement from the circuit confirming the news said that hosting the US Grand Prix had proven financially unviable.
Laguna had been the only North America MotoGP event when it joined the schedule in 2005.
A second US race, at Indianapolis, Indiana, was added in 2008 before the Circuit of the Americas in Austin, Texas, made it three this season.
Indianapolis and Austin are both expected to continue next year as part of an expanded schedule that will include races in Argentina and Brazil.
“For 25 years, Laguna Seca has served as the ‘home' for grand prix motorcycle racing in the United States, having staged 15 world championship grands prix since 1988,” read the statement from Laguna Seca.
“Unfortunately, and in spite of loyal support from fans, sponsors and media, there will not be a 16th US Grand Prix in 2014.
“At this time, the US is only able to support two MotoGP events.
“The support provided by the states of Texas and Indiana make it difficult for us, as a not-for-profit, to currently compete.
“Our pledge to you is to work diligently to return the MotoGP World Championship to Monterey, Calif., in the very near future.”
Laguna's short time on the MotoGP schedule has provided a string of memorable moments, stretching from local rider Nicky Hayden's victories in 2005 and 2006, through the famous Valentino Rossi versus Casey Stoner battle in 2008 and Marc Marquez's rookie win this July.