The dirt track charity event, organised by circuit owner Tony Stewart, will be run on June 8, with 28 drivers in the field.
Ambrose will represent the Children's Medical Centre of Dallas in the race, alongside ‘team-mates' Stewart, Tony Kanaan, Matt Kenseth, Brian Vickers, Aric Almirola and Dave Blaney.
Last year's winner, five-time NASCAR Sprint Cup Champion Jimmie Johnson will also compete in the race, alongside fellow NASCAR stars Denny Hamlin, Kyle Busch, Kasey Kahne, Bobby Labonte, David Reutimann, Carl Edwards, Clint Bowyer and Joey Logano.
Ambrose finished 15th in last year's event, and has tested his 2011 ride at Chattanooga, Tennessee.
This weekend, Marcos Ambrose Motorsport driver George Miedecke will return to the UARA Stars Series after scoring a second place finish on debut in the ARCA Series at New Jersey Motorsport Park last weekend.
Ambrose said he was impressed by his driver's performance, after the former V8 Utes driver led 40 of the 67 laps, only to be passed for the win on the final tour by former Champ Car driver Andrew Ranger.
“The form that George has shown in Late Model competition and now in ARCA will definitely get people talking,” said Ambrose.
See below for video of Ambrose practicing refining dirt track skills