Brno will remain on the MotoGP calendar until at least 2020 after the five-year extension was officially approved.
The outcome follows an agreement that was struck last August between Dorna and the Czech Grand Prix Association.
The Brno circuit in the Czech Republic first staged a world championship grand prix in 1965 and has been on the calendar every year since the early 1990's.
Dorno chief executive Carmelo Ezpeleta, the Czech GP Association Brno mayor Petr Vokral and the president of the South Moravian Region, Michal Hasek have signed the fresh agreement.
“The signing of this agreement is one of the most important acts as far as the MotoGP World Championship is concerned,” Ezpeleta said.
“It is very important for our company, which arranges those races, that the Brno circuit is a part of the championship for the following years.”